Entry Detail


General Information

Database ID:exR0257396
RNA Name:hsa-miR-3652
RNA Type:miRNA
Chromosome:chr12
Starnd:+
Coordinate:
Start Site(bp):103930425End Site(bp):103930442
External Links:hsa-miR-3652



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ATP5MC3
chr2
175176258
175184607
-
GANAB
chr11
62624826
62646726
-
HDLBP
chr2
241227264
241317061
-
PGRMC1
chrX
119236245
119244466
+
PTMA
chr2
231706895
231713541
+
RAB15
chr14
64945814
64973226
-
AC099811.2
chr17
42119674
42154916
-
AP2A1
chr19
49766968
49807113
+
AP2M1
chr3
184174689
184184091
+
ARHGAP33
chr19
35774532
35788822
+
BDNF
chr11
27654893
27722058
-
BRI3
chr7
98252379
98310441
+
CCT6A
chr7
56051685
56063989
+
FKBP8
chr19
18531751
18544077
-
GATC
chr12
120446444
120463749
+
GDF11
chr12
55743122
55757264
+
HLA-DRA
chr6
32439878
32445046
+
HSP90B1
chr12
103930107
103953645
+
MIB2
chr1
1615415
1630610
+
RAB5C
chr17
42124976
42155044
-
SF3A2
chr19
2236824
2248655
+
SFPQ
chr1
35176378
35193145
-
WASF1
chr6
110099819
110180004
-
WBP11
chr12
14784582
14803486
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC022819.1
chr15
99157231
99161140
-
AL591742.2
chr1
114032393
114034511
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.