Entry Detail


General Information

Database ID:exR0257397
RNA Name:hsa-miR-3654
RNA Type:miRNA
Chromosome:chr7
Starnd:-
Coordinate:
Start Site(bp):133034860End Site(bp):133034878
External Links:hsa-miR-3654



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
TUBB
chr6
30720352
30725426
+
DEPDC1B
chr5
60596912
60700190
-
ERI2
chr16
20780193
20900349
-
CCT7
chr2
73233420
73253021
+
APLP2
chr11
130068147
130144811
+
MRPL11
chr11
66435075
66466738
-
HSPA8
chr11
123057489
123063230
-
GGH
chr8
63015079
63038806
-
MDK
chr11
46380756
46383837
+
EGR1
chr5
138465479
138469303
+
MKRN1
chr7
140453033
140479536
-
RPS14
chr5
150442635
150449739
-
DDTL
chr22
23966888
23972556
+
DCAF8
chr1
160215715
160262549
-
RNPS1
chr16
2253116
2268397
-
DCPS
chr11
126304060
126350005
+
MPRIP
chr17
17042545
17217679
+
RPL12
chr9
127447674
127451406
-
RPL13A
chr19
49487554
49492308
+
RPS12
chr6
132814569
132817564
+
HLA-DRA
chr6
32439878
32445046
+
STMN1
chr1
25884181
25906991
-
RPS3
chr11
75399515
75422280
+
RPL3
chr22
39312882
39320389
-
FTH1
chr11
61959718
61967634
-
TSPAN18
chr11
44726465
44932423
+
HLA-DRB5
chr6
32517353
32530287
-
NOLC1
chr10
102152176
102163871
+
HMGA1
chr6
34236873
34246231
+
CCDC85C
chr14
99500190
99604207
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AD000090.1
chr19
35557956
35581954
+
BCDIN3D-AS1
chr12
49827913
49841143
+
LINC00996
chr7
150433654
150448140
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.