Entry Detail


General Information

Database ID:exR0257484
RNA Name:hsa-miR-377-3p
RNA Type:miRNA
Chromosome:chr14
Starnd:+
Coordinate:
Start Site(bp):101062094End Site(bp):101062115
External Links:hsa-miR-377-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ATP9A
chr20
51596514
51768390
-
CLDN11
chr3
170418865
170454733
+
ETS1
chr11
128458761
128587558
-
GPR180
chr13
94601857
94634661
+
IGF1R
chr15
98648539
98964530
+
IRX3
chr16
54283304
54286787
-
MET
chr7
116672196
116798386
+
PALLD
chr4
168497052
168928457
+
PTBP3
chr9
112217716
112333667
-
RNMT
chr18
13726660
13764558
+
RUNX1
chr21
34787801
36004667
-
USP31
chr16
23061406
23149270
-
WDR82
chr3
52254434
52288020
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000712
chr16
68208276
68225678
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC016876.2
chr17
7581964
7584086
-
AC021078.1
chr5
149494314
149504670
-
AL137129.1
chr14
61734138
61776260
+
AL356488.2
chr1
109100193
109100619
+
CKMT2-AS1
chr5
81201341
81301565
-
KCNQ1OT1
chr11
2608328
2699994
-
MIR17HG
chr13
91347820
91354579
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
SNHG1
chr11
62851984
62855953
-
SNHG16
chr17
76557764
76565348
+
SNHG5
chr6
85650491
85678932
-
STAG3L5P-PVRIG2P-PILRB
chr7
100336104
100367831
+
VPS9D1-AS1
chr16
89711856
89718165
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.