Entry Detail


General Information

Database ID:exR0257497
RNA Name:hsa-miR-379-5p
RNA Type:miRNA
Chromosome:chr14
Starnd:+
Coordinate:
Start Site(bp):101022071End Site(bp):101022091
External Links:hsa-miR-379-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ATF7
chr12
53507856
53626410
-
CBX5
chr12
54230942
54280133
-
CHP1
chr15
41230839
41281887
+
DCUN1D4
chr4
51843000
51916837
+
EDA2R
chrX
66595637
66639298
-
MAP3K21
chr1
233327724
233385148
+
NONO
chrX
71283192
71301168
+
NRARP
chr9
137299631
137302271
-
PLD3
chr19
40348456
40380439
+
SIN3A
chr15
75369379
75455842
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000741
chr17
7402357
7402810
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
NEAT1
chr11
65422774
65445540
+
SCARNA9
chr11
93721513
93721865
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.