Entry Detail


General Information

Database ID:exR0257503
RNA Name:hsa-miR-382-5p
RNA Type:miRNA
Chromosome:chr14
Starnd:+
Coordinate:
Start Site(bp):101054316End Site(bp):101054337
External Links:hsa-miR-382-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
APCDD1
chr18
10454635
10489949
+
BCAS2
chr1
114567557
114581629
-
BCL2L11
chr2
111119378
111168445
+
CAMTA1
chr1
6785454
7769706
+
COBLL1
chr2
164653624
164843679
-
ELK1
chrX
47635521
47650604
-
FOSL1
chr11
65892049
65900573
-
IGF2BP3
chr7
23310209
23470491
-
KMT5A
chr12
123383773
123409353
+
MAPRE2
chr18
34976928
35143470
+
NID1
chr1
235975830
236065109
-
PARM1
chr4
74933095
75050115
+
PDE8A
chr15
84980440
85139145
+
RAB18
chr10
27504174
27542237
+
SCAF11
chr12
45919131
45992120
-
SH3GL1
chr19
4360370
4400547
-
STEAP3
chr2
119223831
119265652
+
STK40
chr1
36339624
36385896
-
TAGLN
chr11
117199370
117207464
+
TUB
chr11
8019244
8106112
+
UBN2
chr7
139230356
139308236
+
WDR4
chr21
42843094
42879568
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC092718.4
chr16
81030770
81031485
+
AC126365.1
chr17
20788071
20789584
+
FGD5-AS1
chr3
14920347
14948424
-
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
TUG1
chr22
30969245
30979395
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.