Entry Detail


General Information

Database ID:exR0257738
RNA Name:hsa-miR-4485-3p
RNA Type:miRNA
Chromosome:chr11
Starnd:-
Coordinate:
Start Site(bp):10508277End Site(bp):10508296
External Links:hsa-miR-4485-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
C11orf24
chr11
68261338
68272001
-
CDK4
chr12
57747727
57756013
-
CKAP5
chr11
46743048
46846308
-
CYB561A3
chr11
61348745
61362299
-
SERINC1
chr6
122443351
122471807
-
NES
chr1
156668763
156677407
-
NDUFB11
chrX
47142216
47145504
-
FUBP1
chr1
77944055
77979110
-
RPL27
chr17
42998273
43002959
+
YEATS2
chr3
183697797
183812624
+
CIRBP
chr19
1259384
1274880
+
DNM1
chr9
128191655
128255248
+
MAP3K11
chr11
65597756
65615382
-
RPL13
chr16
89560657
89566828
+
EXOSC6
chr16
70246778
70251940
-
SBF2
chr11
9778667
10294207
-
HSPA8
chr11
123057489
123063230
-
RPS27A
chr2
55231903
55235853
+
EIF3F
chr11
7970251
8001862
+
RNASEH2C
chr11
65714005
65720818
-
FAU
chr11
65120630
65122177
-
HSP90AB1
chr6
44246166
44253888
+
RPS3
chr11
75399515
75422280
+
NR3C1
chr5
143277931
143435512
-
SPG11
chr15
44562696
44663678
-
MTRNR2L6
chr7
142666272
142667718
+
LGR4
chr11
27365961
27472790
-
FRMD8
chr11
65386621
65413525
+
CRTC3
chr15
90529923
90645345
+
MTRNR2L12
chr3
96617188
96618236
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
MALAT1
chr11
65497688
65506516
+
XIST
chrX
73820649
73852723
-
AD000090.1
chr19
35557956
35581954
+
LINC01002
chr19
198264
246544
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.