Entry Detail


General Information

Database ID:exR0257745
RNA Name:hsa-miR-4492
RNA Type:miRNA
Chromosome:chr11
Starnd:+
Coordinate:
Start Site(bp):118910765End Site(bp):118910781
External Links:hsa-miR-4492



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
AGFG1
chr2
227472152
227561214
+
NRARP
chr9
137299631
137302271
-
RHOA
chr3
49359145
49412998
-
AATF
chr17
36948954
37056871
+
ANOS1
chrX
8528874
8732137
-
DDRGK1
chr20
3190350
3204685
-
FBXL3
chr13
76992598
77027195
-
LEPR
chr1
65420652
65641559
+
MDP1
chr14
24213943
24216070
-
MELTF
chr3
196988621
197029817
-
NCKAP5L
chr12
49791146
49828750
-
PKN3
chr9
128702503
128720916
+
RNF149
chr2
101271219
101308701
-
SH3BP2
chr4
2793071
2841098
+
SNAPC2
chr19
7920338
7923250
+
SOCS2
chr12
93569814
93583487
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AL096870.2
chr14
24209646
24215987
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.