Entry Detail


General Information

Database ID:exR0257772
RNA Name:hsa-miR-450b-5p
RNA Type:miRNA
Chromosome:chrX
Starnd:-
Coordinate:
Start Site(bp):134540231End Site(bp):134540252
External Links:hsa-miR-450b-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
CPSF2
chr14
92121969
92172145
+
DUSP14
chr17
37489891
37513501
+
MEF2A
chr15
99565417
99716466
+
VCP
chr9
35056064
35072627
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000540
chr14
55799534
55821940
+
hsa_circ_0000418
chr12
69983264
69985939
+
hsa_circ_0001492
chr5
65284462
65290692
+
hsa_circ_0000284
chr11
33307958
33309057
+
hsa_circ_0000494
chr13
76134888
76143643
+
hsa_circ_0000943
chr19
47421744
47440665
+
hsa_circ_0000398
chr12
46633461
46637097
-
hsa_circ_0001168
chr20
47691321
47707559
+
hsa_circ_0001263
chr3
5215701
5216099
+
hsa_circ_0000799
chr17
65941524
65972074
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
A1BG-AS1
chr19
58347718
58355455
+
AC005034.3
chr2
75697583
75697996
+
AC010327.5
chr19
55216660
55221616
+
AC105339.2
chr15
82752884
82757208
+
AL049795.2
chr1
32170733
32176568
+
AL137782.1
chr13
75549773
75807120
+
FGD5-AS1
chr3
14920347
14948424
-
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
SNHG1
chr11
62851984
62855953
-
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.