Entry Detail


General Information

Database ID:exR0257817
RNA Name:hsa-miR-455-5p
RNA Type:miRNA
Chromosome:chr9
Starnd:+
Coordinate:
Start Site(bp):114209449End Site(bp):114209470
External Links:hsa-miR-455-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
AEN
chr15
88621337
88632281
+
BCL3
chr19
44747705
44760044
+
CANT1
chr17
78991716
79009867
-
NAPA
chr19
47487637
47515091
-
NRIP1
chr21
14961235
15065936
-
RCCD1
chr15
90954870
90963125
+
REEP4
chr8
22138020
22141951
-
RGS4
chr1
163068775
163076802
+
UBE2K
chr4
39698109
39782792
+
VCL
chr10
73995193
74121363
+
WDR5B
chr3
122411846
122416062
-
ZBTB34
chr9
126860665
126885878
+
ZNF10
chr12
133130575
133159465
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC020928.2
chr19
36773712
36775908
-
AL034397.3
chrX
66015461
66020422
+
GAS5
chr1
173858559
173868882
-
KCNQ1OT1
chr11
2608328
2699994
-
MALAT1
chr11
65497688
65506516
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.