exRNAdisease

Entry Detail

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General Information

Database ID: exR0257940 RNA Name: hsa-miR-4709-3p RNA Type: miRNA Chromosome: chr14 Starnd: - Coordinate: Start Site(bp): 74480135 End Site(bp): 74480157 External Links: hsa-miR-4709-3p

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand BCORL1 chrX 129981107 130058083 + COQ8A chr1 226897536 226987545 + DNAJB6 chr7 157335381 157417439 + LAMC1 chr1 183023420 183145592 + PURA chr5 140107777 140125619 + TMEM167A chr5 83052846 83077863 - BIK chr22 43110750 43129712 + CLSTN3 chr12 7129698 7158945 + DHX16 chr6 30653119 30673006 - EMC4 chr15 34225013 34230156 + ETV3 chr1 157121191 157138474 - GATM chr15 45361124 45402327 - MED1 chr17 39404285 39451272 - MED28 chr4 17614641 17634105 + MSH3 chr5 80654652 80876815 + MTRNR2L12 chr3 96617188 96618236 - NDUFA4 chr7 10931943 10940153 - NPC2 chr14 74476192 74494177 - PLBD1 chr12 14503661 14567883 - PLEKHA5 chr12 19129752 19376400 + RACK1 chr5 181236897 181248096 - RBM15B chr3 51391285 51397908 + SLC50A1 chr1 155135344 155138857 + TPRG1L chr1 3625015 3630127 + WNK1 chr12 752579 911452 + miRNA targets: NA circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.