Entry Detail


General Information

Database ID:exR0257941
RNA Name:hsa-miR-4709-5p
RNA Type:miRNA
Chromosome:chr14
Starnd:-
Coordinate:
Start Site(bp):74480175End Site(bp):74480196
External Links:hsa-miR-4709-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
BCORL1
chrX
129981107
130058083
+
CBX2
chr17
79778148
79787983
+
CTNND1
chr11
57753243
57819546
+
DNAJB6
chr7
157335381
157417439
+
ERP29
chr12
112013348
112023449
+
LAMC1
chr1
183023420
183145592
+
ARID4B
chr1
235131634
235328219
-
CLSTN3
chr12
7129698
7158945
+
DHX16
chr6
30653119
30673006
-
GFM2
chr5
74721206
74767147
-
HNRNPL
chr19
38836388
38852347
-
HOXB13
chr17
48724763
48729178
-
HSP90AA1
chr14
102080738
102139699
-
IDH1
chr2
208236227
208266074
-
IREB2
chr15
78437431
78501453
+
MKKS
chr20
10401009
10434222
-
MSH3
chr5
80654652
80876815
+
NPC2
chr14
74476192
74494177
-
PABPC1
chr8
100685816
100722809
-
PODXL
chr7
131500262
131558217
-
PRDX5
chr11
64318121
64321811
+
SCAF8
chr6
154733325
154834244
+
TIAM2
chr6
154832697
155257723
+
UGP2
chr2
63840940
63891562
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC005479.1
chr14
74474007
74474864
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.