Entry Detail


General Information

Database ID:exR0258117
RNA Name:hsa-miR-492
RNA Type:miRNA
Chromosome:chr12
Starnd:+
Coordinate:
Start Site(bp):94834427End Site(bp):94834449
External Links:hsa-miR-492



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
DYRK1A
chr21
37365573
37526358
+
KMT5A
chr12
123383773
123409353
+
NASP
chr1
45583846
45618904
+
ACAA2
chr18
49782164
49813953
-
ARL6IP1
chr16
18791669
18801572
-
EPCAM
chr2
47345158
47387601
+
GOPC
chr6
117560269
117602542
-
KRT19
chr17
41523617
41528308
-
RPS12
chr6
132814569
132817564
+
RPS16
chr19
39433137
39435949
-
SLC18B1
chr6
132769370
132813339
-
SLC20A1
chr2
112645939
112663825
+
TRMT44
chr4
8436140
8493531
+
UBC
chr12
124911604
124917368
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:NA
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.