Entry Detail


General Information

Database ID:exR0258118
RNA Name:hsa-miR-493-3p
RNA Type:miRNA
Chromosome:chr14
Starnd:+
Coordinate:
Start Site(bp):100869116End Site(bp):100869137
External Links:hsa-miR-493-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ANKS1A
chr6
34889255
35091406
+
ASF1B
chr19
14119512
14136613
-
BACH2
chr6
89926528
90296908
-
BRPF3
chr6
36196744
36232790
+
C17orf49
chr17
7014495
7017525
+
DYNLRB1
chr20
34516409
34540958
+
EPG5
chr18
45847609
45967329
-
HIF1AN
chr10
100529072
100559998
+
MEX3A
chr1
156072013
156082465
-
MFN2
chr1
11980181
12013514
+
MMGT1
chrX
135962070
135974063
-
MTUS1
chr8
17643795
17800917
-
NPTX1
chr17
80466834
80477843
-
PRKAR2A
chr3
48744597
48847874
-
PXN
chr12
120210439
120265771
-
RNASEK-C17orf49
chr17
7012635
7017520
+
XPO6
chr16
28097979
28211920
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001146
chr20
34241449
34246936
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC016831.1
chr7
130876809
130913310
-
AC016876.2
chr17
7581964
7584086
-
AC022167.2
chr16
8848105
8860456
+
AC144548.1
chr12
110387463
110445548
-
AL359924.1
chr1
237862175
237928321
+
MIR200CHG
chr12
6963246
6964447
+
TUG1
chr22
30969245
30979395
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.