Entry Detail


General Information

Database ID:exR0258162
RNA Name:hsa-miR-502-3p
RNA Type:miRNA
Chromosome:chrX
Starnd:+
Coordinate:
Start Site(bp):50014649End Site(bp):50014670
External Links:hsa-miR-502-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
CDC23
chr5
138187650
138213343
-
CRIM1
chr2
36355778
36551135
+
IL21R
chr16
27402174
27452042
+
JADE1
chr4
128809700
128875224
+
MAP3K21
chr1
233327724
233385148
+
MGAT3
chr22
39457012
39492194
+
MTMR12
chr5
32226994
32312987
-
NR1D2
chr3
23945286
23980617
+
NRIP1
chr21
14961235
15065936
-
PHTF2
chr7
77798792
77957503
+
SETD7
chr4
139495941
139606699
-
SH3PXD2A
chr10
103594027
103855543
-
TNRC6B
chr22
40044817
40335808
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
GAS5
chr1
173858559
173868882
-
LINC00641
chr14
21200079
21206900
-
MIR17HG
chr13
91347820
91354579
+
NEAT1
chr11
65422774
65445540
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.