Entry Detail


General Information

Database ID:exR0258168
RNA Name:hsa-miR-5047
RNA Type:miRNA
Chromosome:chr17
Starnd:-
Coordinate:
Start Site(bp):64501230End Site(bp):64501250
External Links:hsa-miR-5047



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
DDX5
chr17
64498254
64508199
-
FKBP4
chr12
2794970
2805423
+
ASF1A
chr6
118894152
118909171
+
GM2A
chr5
151212150
151270440
+
U2AF2
chr19
55654146
55674715
+
YWHAQ
chr2
9583967
9630997
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001225
chr22
36684772
36684943
-
hsa_circ_0001880
chr9
110084263
110087285
+
hsa_circ_0000018
chr1
15860731
15863309
+
hsa_circ_0000799
chr17
65941524
65972074
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC015871.3
chr15
79920195
79922455
-
AC018521.1
chr17
47945424
47981736
+
AC025171.4
chr5
43061395
43062441
+
AC067930.1
chr8
143579636
143580670
+
AC245033.4
chr15
82533175
82540008
-
DANCR
chr4
52712404
52720351
+
JPX
chrX
73944182
74070408
+
KCNQ1OT1
chr11
2608328
2699994
-
LINC00894
chrX
149938613
150224580
+
MUC20-OT1
chr3
195658062
195739964
+
NEAT1
chr11
65422774
65445540
+
NNT-AS1
chr5
43571594
43603230
-
SNHG12
chr1
28578538
28583132
-
SNHG16
chr17
76557764
76565348
+
ZFAS1
chr20
49278178
49299600
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.