Entry Detail


General Information

Database ID:exR0258188
RNA Name:hsa-miR-5094
RNA Type:miRNA
Chromosome:chr15
Starnd:-
Coordinate:
Start Site(bp):89850690End Site(bp):89850711
External Links:hsa-miR-5094



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ZNHIT3
chr17
36486629
36499310
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001454
chr4
154524454
154533552
+
hsa_circ_0001005
chr2
53921020
53978078
-
hsa_circ_0000384
chr12
27867712
27877119
+
hsa_circ_0001159
chr20
40161688
40179999
-
hsa_circ_0000698
chr16
47531309
47549512
+
hsa_circ_0001445
chr4
144464661
144465125
+
hsa_circ_0000943
chr19
47421744
47440665
+
hsa_circ_0001667
chr7
810107
814799
+
hsa_circ_0001376
chr3
194169185
194175121
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC074117.1
chr2
27356246
27367622
+
AL356488.2
chr1
109100193
109100619
+
AL359091.1
chr9
128392007
128393510
-
DSCAM-AS1
chr21
40383083
40385358
+
FTX
chrX
73940435
74293574
-
GAS5
chr1
173858559
173868882
-
NEAT1
chr11
65422774
65445540
+
NUTM2B-AS1
chr10
79661394
79826594
-
PDCD4-AS1
chr10
110869868
110872233
-
SNHG3
chr1
28505980
28510892
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.