exRNAdisease

Entry Detail

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General Information

Database ID: exR0258191 RNA Name: hsa-miR-5100 RNA Type: miRNA Chromosome: chr10 Starnd: + Coordinate: Start Site(bp): 42997630 End Site(bp): 42997651 External Links: hsa-miR-5100

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand SNU13 chr22 41673933 41690504 - GHITM chr10 84139509 84153568 + AGRN chr1 1020120 1056118 + PRRC2B chr9 131373636 131500197 + HNRNPH1 chr5 179614178 179634784 - GTF3C4 chr9 132670035 132694953 + PPCDC chr15 75023586 75117462 + SLC35B1 chr17 49700934 49709014 - SRP72 chr4 56467617 56503681 + SRPK1 chr6 35832966 35921342 - CHPF chr2 219538948 219543809 - NOP14 chr4 2937933 2963406 - SLC52A2 chr8 144354135 144361272 + THOP1 chr19 2785503 2815807 + MACF1 chr1 39081316 39487177 + CPTP chr1 1324756 1328896 + UBR4 chr1 19074510 19210266 - ZNF136 chr19 12163064 12189871 + HCN1 chr5 45254948 45696498 - NUP93 chr16 56730118 56850286 + KCTD2 chr17 75032575 75065889 + NIN chr14 50719763 50831121 - KDSR chr18 63327726 63367228 - ATP1A1 chr1 116372668 116410261 + PRMT6 chr1 107056674 107067636 + FLNA chrX 154348524 154374638 - NUP107 chr12 68686951 68745809 + P4HTM chr3 48989889 49007153 + VSIG10 chr12 118063593 118136026 - WASF2 chr1 27404230 27490167 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand POLR2J4 chr7 43940895 44019175 - SNHG3 chr1 28505980 28510892 + AC008738.2 chr19 33305036 33309387 + Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.