Entry Detail


General Information

Database ID:exR0258244
RNA Name:hsa-miR-519a-3p
RNA Type:miRNA
Chromosome:chr19
Starnd:+
Coordinate:
Start Site(bp):53762397End Site(bp):53762418
External Links:hsa-miR-519a-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ARL4A
chr7
12686856
12690958
+
CAMK2N1
chr1
20482391
20486210
-
CCDC93
chr2
117915478
118014133
-
CCSAP
chr1
229321011
229343294
-
CDK6
chr7
92604921
92836594
-
CIT
chr12
119685791
119877320
-
CLCN6
chr1
11806096
11848079
+
EEA1
chr12
92770637
92929331
-
EIF5A2
chr3
170888418
170908644
-
FAM13A
chr4
88725955
89111398
-
FYCO1
chr3
45917899
45995824
-
LDLRAD3
chr11
35943981
36232136
+
LONP2
chr16
48244300
48363122
+
MAGI3
chr1
113390515
113685923
+
MBP
chr18
76978827
77133683
-
NAA16
chr13
41311267
41377030
+
OCRL
chrX
129539849
129592561
+
PDRG1
chr20
31944337
31952046
-
PLAGL2
chr20
32192504
32207743
-
RGMA
chr15
93035273
93089204
-
SEC16A
chr9
136440096
136483759
-
SLC16A14
chr2
230034982
230068993
-
SLC46A3
chr13
28700064
28718970
-
SLC7A2
chr8
17497088
17570573
+
SMAD7
chr18
48919853
48952052
-
SYAP1
chrX
16719612
16765340
+
TAOK3
chr12
118149801
118372907
-
ZFAND4
chr10
45615500
45672780
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001495
chr5
68470703
68471364
+
hsa_circ_0000494
chr13
76134888
76143643
+
hsa_circ_0000018
chr1
15860731
15863309
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC002094.1
chr17
28357647
28381697
+
AC010542.4
chr16
66549280
66551189
+
AC015871.3
chr15
79920195
79922455
-
AC021078.1
chr5
149494314
149504670
-
AC021092.1
chr19
44103007
44113183
-
AC024940.6
chr12
31280422
31280895
-
AC087477.2
chr15
96354237
96405235
+
AC243964.3
chr19
44631573
44725217
-
AL137782.1
chr13
75549773
75807120
+
AL158206.1
chr9
19453209
19455173
+
CKMT2-AS1
chr5
81201341
81301565
-
EPB41L4A-AS1
chr5
112160526
112164818
+
ERICD
chr8
140636281
140638283
+
H19
chr11
1995176
2001470
-
HAGLR
chr2
176164051
176188958
-
HOTAIR
chr12
53962308
53974956
-
MAGI2-AS3
chr7
79452877
79471208
+
MIR17HG
chr13
91347820
91354579
+
NEAT1
chr11
65422774
65445540
+
STAG3L5P-PVRIG2P-PILRB
chr7
100336104
100367831
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.