Entry Detail


General Information

Database ID:exR0258256
RNA Name:hsa-miR-520b-3p
RNA Type:miRNA
Chromosome:chr19
Starnd:+
Coordinate:
Start Site(bp):53701267End Site(bp):53701287
External Links:hsa-miR-520b-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
TNS1
chr2
217799588
218033982
-
CLCN6
chr1
11806096
11848079
+
SIRPA
chr20
1894167
1940592
+
CELF2
chr10
10798397
11336675
+
MBP
chr18
76978827
77133683
-
PLAGL2
chr20
32192504
32207743
-
APP
chr21
25880550
26171128
-
KLHL28
chr14
44924319
45042322
-
SEC16A
chr9
136440096
136483759
-
CCDC93
chr2
117915478
118014133
-
MAGI3
chr1
113390515
113685923
+
PTP4A1
chr6
63521746
63583436
+
SYAP1
chrX
16719612
16765340
+
SEMA3C
chr7
80742538
80922359
-
ADAR
chr1
154582057
154628013
-
CUL1
chr7
148697914
148801110
+
CHD9
chr16
53055033
53329150
+
TRIB1
chr8
125430358
125438403
+
SLC16A14
chr2
230034982
230068993
-
IFNAR1
chr21
33324429
33359864
+
WDR82
chr3
52254434
52288020
-
RUNDC1
chr17
42980565
42993690
+
NPAS2
chr2
100820139
100996829
+
JPT1
chr17
75135248
75168281
-
LONP2
chr16
48244300
48363122
+
OCRL
chrX
129539849
129592561
+
ZFAND4
chr10
45615500
45672780
-
PHC3
chr3
170086732
170181749
-
ATXN1
chr6
16299112
16761491
-
FAM177A1
chr14
35044907
35113130
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AL049840.4
chr14
103694516
103695050
-
ARHGAP27P1-BPTFP1-KPNA2P3
chr17
64749663
64781707
-
FGD5-AS1
chr3
14920347
14948424
-
MIR17HG
chr13
91347820
91354579
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
SNHG16
chr17
76557764
76565348
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.