exRNAdisease

Entry Detail

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General Information

Database ID: exR0258257 RNA Name: hsa-miR-520c-3p RNA Type: miRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 53707506 End Site(bp): 53707527 External Links: hsa-miR-520c-3p

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand PDRG1 chr20 31944337 31952046 - NAA16 chr13 41311267 41377030 + IFNAR1 chr21 33324429 33359864 + FYCO1 chr3 45917899 45995824 - MSL1 chr17 40121971 40136917 + ZFAND4 chr10 45615500 45672780 - CLCN6 chr1 11806096 11848079 + SYAP1 chrX 16719612 16765340 + AMPD2 chr1 109616104 109632053 + EIF5A2 chr3 170888418 170908644 - PTP4A1 chr6 63521746 63583436 + TNS1 chr2 217799588 218033982 - CAMK2N1 chr1 20482391 20486210 - RPP14 chr3 58306247 58324695 + PHC3 chr3 170086732 170181749 - HTD2 chr3 58306262 58318574 + MBP chr18 76978827 77133683 - SLC7A2 chr8 17497088 17570573 + TRIB1 chr8 125430358 125438403 + SIRPA chr20 1894167 1940592 + CCSAP chr1 229321011 229343294 - SRRT chr7 100875103 100888664 + CHD9 chr16 53055033 53329150 + FAM177A1 chr14 35044907 35113130 + PLAGL2 chr20 32192504 32207743 - CLIP4 chr2 29097705 29189643 + NPAS2 chr2 100820139 100996829 + LONP2 chr16 48244300 48363122 + KLHL28 chr14 44924319 45042322 - APP chr21 25880550 26171128 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000943 chr19 47421744 47440665 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL049840.4 chr14 103694516 103695050 - ARHGAP27P1-BPTFP1-KPNA2P3 chr17 64749663 64781707 - FGD5-AS1 chr3 14920347 14948424 - MIR17HG chr13 91347820 91354579 + NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - SNHG16 chr17 76557764 76565348 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.