Entry Detail


General Information

Database ID:exR0258260
RNA Name:hsa-miR-520d-5p
RNA Type:miRNA
Chromosome:chr19
Starnd:+
Coordinate:
Start Site(bp):53720111End Site(bp):53720130
External Links:hsa-miR-520d-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
AFF3
chr2
99545419
100192428
-
KLHL28
chr14
44924319
45042322
-
SIK2
chr11
111602449
111730855
+
UBE2J1
chr6
89326625
89352722
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000095
chr1
95609446
95616975
+
hsa_circ_0000284
chr11
33307958
33309057
+
hsa_circ_0001146
chr20
34241449
34246936
-
hsa_circ_0000799
chr17
65941524
65972074
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC008738.7
chr19
33207129
33207639
+
AC009022.1
chr16
69976297
70065948
-
AC021078.1
chr5
149494314
149504670
-
AC024940.6
chr12
31280422
31280895
-
AC068768.1
chr12
123262060
123262402
+
AC125257.1
chr17
41848518
41851447
-
AC239868.1
chr1
149861271
149862504
+
AL031595.3
chr22
44139365
44153626
+
AL137129.1
chr14
61734138
61776260
+
FGD5-AS1
chr3
14920347
14948424
-
GAS5
chr1
173858559
173868882
-
MCM3AP-AS1
chr21
46229196
46259390
+
MIR29B2CHG
chr1
207801518
207879115
-
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
PITPNA-AS1
chr17
1516931
1518096
+
SCARNA9
chr11
93721513
93721865
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.