exRNAdisease

Entry Detail

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General Information

Database ID: exR0258274 RNA Name: hsa-miR-525-3p RNA Type: miRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 53697584 End Site(bp): 53697605 External Links: hsa-miR-525-3p

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ANKRD52 chr12 56237807 56258384 - UCHL1 chr4 41256413 41268455 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001588 chr6 26204839 26205043 + hsa_circ_0000540 chr14 55799534 55821940 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand NORAD chr20 36045618 36051018 - Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.