Entry Detail


General Information

Database ID:exR0258275
RNA Name:hsa-miR-525-5p
RNA Type:miRNA
Chromosome:chr19
Starnd:+
Coordinate:
Start Site(bp):53697547End Site(bp):53697567
External Links:hsa-miR-525-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
C4orf46
chr4
158666675
158672255
-
CA12
chr15
63321378
63381846
-
CELSR2
chr1
109249539
109275751
+
CHD1
chr5
98853985
98928957
-
HMOX1
chr22
35380361
35394207
+
ITGA5
chr12
54395261
54419266
-
KIAA0232
chr4
6781375
6884170
+
LRP8
chr1
53242364
53328469
-
NCEH1
chr3
172630249
172711218
-
ORMDL3
chr17
39921041
39927601
-
PRRC2B
chr9
131373636
131500197
+
SORBS3
chr8
22544986
22575788
+
SSH2
chr17
29625938
29930276
-
TGOLN2
chr2
85318020
85328296
-
USP16
chr21
29024629
29054488
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000892
chr19
11941431
12014514
+
hsa_circ_0001361
chr3
171830241
171851336
+
hsa_circ_0001146
chr20
34241449
34246936
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC079781.5
chr7
97851688
97972985
-
AL049543.1
chr6
28587378
28591747
+
AL049795.2
chr1
32170733
32176568
+
GAS5
chr1
173858559
173868882
-
H19
chr11
1995176
2001470
-
HCP5
chr6
31463170
31478936
+
MIRLET7BHG
chr22
46053869
46113928
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
XIST
chrX
73820649
73852723
-
ZFAS1
chr20
49278178
49299600
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.