Entry Detail


General Information

Database ID:exR0258280
RNA Name:hsa-miR-532-3p
RNA Type:miRNA
Chromosome:chrX
Starnd:+
Coordinate:
Start Site(bp):50003204End Site(bp):50003225
External Links:hsa-miR-532-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ABHD2
chr15
89087459
89202355
+
ABL2
chr1
179099330
179229684
-
CHD2
chr15
92900189
93027996
+
FOSL2
chr2
28392448
28417317
+
HNRNPH1
chr5
179614178
179634784
-
KIAA1522
chr1
32741830
32774970
+
NPTN
chr15
73560014
73634134
-
PACS1
chr11
66070272
66244744
+
PHC3
chr3
170086732
170181749
-
SIAH2
chr3
150741125
150763477
-
SLC7A6
chr16
68264516
68301823
+
SMG5
chr1
156249224
156282825
-
ZHX3
chr20
41178448
41317672
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AL132655.2
chr20
58817132
58817725
-
KCNQ1OT1
chr11
2608328
2699994
-
LZTS1-AS1
chr8
20275773
20290458
+
MALAT1
chr11
65497688
65506516
+
MIR34AHG
chr1
9148011
9198906
-
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
SNHG10
chr14
95521943
95534889
-
VPS9D1-AS1
chr16
89711856
89718165
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.