Entry Detail


General Information

Database ID:exR0258291
RNA Name:hsa-miR-545-3p
RNA Type:miRNA
Chromosome:chrX
Starnd:-
Coordinate:
Start Site(bp):74287126End Site(bp):74287147
External Links:hsa-miR-545-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ANKRD50
chr4
124664048
124712732
-
ARRB2
chr17
4710596
4721499
+
BVES
chr6
105096822
105137157
-
CREBRF
chr5
173056352
173139284
+
FAM89A
chr1
231018958
231040254
-
NAV1
chr1
201622885
201826969
+
SPECC1
chr17
20009344
20319026
+
TOB2
chr22
41433494
41446801
-
TRIM33
chr1
114392790
114511203
-
UTRN
chr6
144285701
144853034
+
WDR4
chr21
42843094
42879568
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001409
chr4
48835417
48859382
+
hsa_circ_0000458
chr12
124071293
124074996
+
hsa_circ_0000943
chr19
47421744
47440665
+
hsa_circ_0001452
chr4
153562079
153574079
-
hsa_circ_0001263
chr3
5215701
5216099
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC016876.2
chr17
7581964
7584086
-
AC114980.1
chr5
93860669
93863825
-
AC125257.1
chr17
41848518
41851447
-
AC245033.4
chr15
82533175
82540008
-
GAS5
chr1
173858559
173868882
-
LINC00476
chr9
95759231
95876049
-
MALAT1
chr11
65497688
65506516
+
MCM3AP-AS1
chr21
46229196
46259390
+
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
OIP5-AS1
chr15
41283990
41309737
+
RRN3P2
chr16
29074976
29116718
+
SNHG1
chr11
62851984
62855953
-
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.