Entry Detail


General Information

Database ID:exR0258464
RNA Name:hsa-miR-574-5p
RNA Type:miRNA
Chromosome:chr4
Starnd:+
Coordinate:
Start Site(bp):38868056End Site(bp):38868078
External Links:hsa-miR-574-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
CCDC88C
chr14
91271323
91417844
-
CARMIL1
chr6
25279078
25620530
+
AHCYL1
chr1
109984765
110023742
+
ARID3A
chr19
925781
975939
+
CHD7
chr8
60678740
60868028
+
IDH3A
chr15
78131498
78171945
+
FAM102A
chr9
127940582
127980989
-
EEF2K
chr16
22206278
22288738
+
LRP8
chr1
53242364
53328469
-
SMCO4
chr11
93478472
93543391
-
ALDOA
chr16
30064164
30070457
+
ZNF621
chr3
40524878
40574685
+
MAP7D1
chr1
36155579
36180849
+
RAB10
chr2
26034084
26137454
+
TACC1
chr8
38728186
38853028
+
KLF11
chr2
10042849
10054836
+
TEAD1
chr11
12674421
12944737
+
MGRN1
chr16
4616493
4690974
+
SLC9A1
chr1
27098809
27166981
-
FNDC3A
chr13
48975912
49209779
+
SLC25A43
chrX
119399336
119454478
+
CTSD
chr11
1752755
1764573
-
PHF20L1
chr8
132775358
132848807
+
DCAF8
chr1
160215715
160262549
-
PIK3R3
chr1
46040140
46133036
-
RHOU
chr1
228735479
228746664
+
GALNT3
chr2
165747588
165794659
-
HOXC8
chr12
54008985
54012769
+
NID1
chr1
235975830
236065109
-
WSB2
chr12
118032694
118062430
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC005394.2
chr19
28435388
28727680
-
AC021092.1
chr19
44103007
44113183
-
AL358472.3
chr1
153966516
153966930
+
DSCAM-AS1
chr21
40383083
40385358
+
NEAT1
chr11
65422774
65445540
+
SNHG7
chr9
136721366
136728184
-
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.