exRNAdisease

Entry Detail

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General Information

Database ID: exR0258485 RNA Name: hsa-miR-588 RNA Type: miRNA Chromosome: chr6 Starnd: + Coordinate: Start Site(bp): 126484646 End Site(bp): 126484666 External Links: hsa-miR-588

Disease Information

Disease Name: Obesity Disease Category: Other Diseases MeSH ID: D009765 Type: Diseases Category/Nutritional and Metabolic Diseases Alias: Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics

Expression Detail

GEO ID: GSE125494 Description: Adipocyte-Derived Exosomal microRNAs in subject serum samples Experimental Design: Disease vs Control Case Disease Type: Obesity Case Disease SubType: NA Case Sample: Obesity Control Sample: Control Number of Case: 8 Number of Control: 3 Number of Samples: 11

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ADGRE5 chr19 14380501 14408725 + ARHGDIA chr17 81867721 81871378 - EDEM3 chr1 184690237 184754907 - EIF1AD chr11 65996545 66002176 - FURIN chr15 90868588 90883458 + GLIS2 chr16 4314761 4339597 + NID1 chr1 235975830 236065109 - NIP7 chr16 69337996 69343106 + NT5DC3 chr12 103770453 103841234 - STK40 chr1 36339624 36385896 - TSPAN14 chr10 80454166 80533124 + TUB chr11 8019244 8106112 + ZBTB39 chr12 56998836 57006546 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001025 chr2 69565029 69565697 - hsa_circ_0000384 chr12 27867712 27877119 + hsa_circ_0001247 chr22 46125304 46136418 + hsa_circ_0000284 chr11 33307958 33309057 + hsa_circ_0000126 chr1 150997086 150999803 + hsa_circ_0000744 chr17 15968183 15971435 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007161.3 chr7 7640752 8004053 + AL022311.1 chr22 37876148 37895563 + AL359924.1 chr1 237862175 237928321 + H19 chr11 1995176 2001470 - MIR497HG chr17 7015818 7019659 - NEAT1 chr11 65422774 65445540 + PINK1-AS chr1 20642657 20652193 - SNHG8 chr4 118278709 118285316 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE125494 Sample Source: Blood Source Fraction: Exosome Platform: GPL21572 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 2546 Sample treatment protocol: To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes. RNA Extract protocol: Total RNA was isolated following mirVana's total RNA Isolation kit. RNA library preparation protocol: biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample

Reference

PMID: 31331347 Title: Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs Author: Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ Journal: J Transl Med. 2019 Jul 22;17(1):232. Description: We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.