Entry Detail


General Information

Database ID:exR0258587
RNA Name:hsa-miR-642a-5p
RNA Type:miRNA
Chromosome:chr19
Starnd:+
Coordinate:
Start Site(bp):45674943End Site(bp):45674964
External Links:hsa-miR-642a-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
AFF4
chr5
132875395
132963634
-
ARID3A
chr19
925781
975939
+
E2F3
chr6
20401879
20493714
+
GFOD1
chr6
13357830
13487662
-
HIF1AN
chr10
100529072
100559998
+
MAP3K12
chr12
53479669
53500063
-
MAPKAPK2
chr1
206684944
206734283
+
MDK
chr11
46380756
46383837
+
PPP6R1
chr19
55229779
55259017
-
SIK2
chr11
111602449
111730855
+
SLC7A2
chr8
17497088
17570573
+
SYVN1
chr11
65121780
65134533
-
TMED10
chr14
75131469
75176612
-
TMEM30B
chr14
61277370
61281761
-
UBR3
chr2
169827454
170084131
+
VAT1
chr17
43014607
43025123
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001005
chr2
53921020
53978078
-
hsa_circ_0001146
chr20
34241449
34246936
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC004832.5
chr22
30420512
30420912
+
AC007952.4
chr17
19112000
19112636
-
AC010327.5
chr19
55216660
55221616
+
AC016876.2
chr17
7581964
7584086
-
AL645465.1
chr1
244375100
244409592
-
MIR34AHG
chr1
9148011
9198906
-
MIR497HG
chr17
7015818
7019659
-
NEAT1
chr11
65422774
65445540
+
NORAD
chr20
36045618
36051018
-
ZFAS1
chr20
49278178
49299600
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.