Entry Detail


General Information

Database ID:exR0258599
RNA Name:hsa-miR-650
RNA Type:miRNA
Chromosome:chr22
Starnd:+
Coordinate:
Start Site(bp):22822791End Site(bp):22822811
External Links:hsa-miR-650



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
POM121C
chr7
75416786
75486299
-
ATXN2L
chr16
28823035
28837237
+
RNF220
chr1
44405194
44651724
+
POLE3
chr9
113407235
113410675
-
SFN
chr1
26863149
26864456
+
SH3GL1
chr19
4360370
4400547
-
STOM
chr9
121338987
121370304
-
HNRNPUL1
chr19
41262496
41307787
+
LMNA
chr1
156082573
156140089
+
SLC9A3R1
chr17
74748628
74769353
+
HPCAL1
chr2
10302889
10427617
+
TRAF7
chr16
2155698
2178129
+
GBF1
chr10
102245532
102382899
+
ZNF623
chr8
143636013
143656418
+
USP22
chr17
20999596
21043760
-
POLDIP3
chr22
42583721
42614962
-
VHL
chr3
10141008
10152220
+
ABL1
chr9
130713016
130887675
+
TSKU
chr11
76782251
76798153
+
TRAPPC10
chr21
44012309
44106552
+
SLC29A1
chr6
44219553
44234142
+
HIF1AN
chr10
100529072
100559998
+
NBL1
chr1
19596979
19658456
+
AFAP1
chr4
7758714
7939926
-
CBFA2T3
chr16
88874858
88977207
-
AKT2
chr19
40230317
40285536
-
RAB1B
chr11
66268590
66277492
+
PAK4
chr19
39125770
39182816
+
DUSP8
chr11
1554051
1572271
-
TESMIN
chr11
68707440
68751520
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001168
chr20
47691321
47707559
+
hsa_circ_0001146
chr20
34241449
34246936
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC068768.1
chr12
123262060
123262402
+
AC118758.3
chr7
56809214
56848800
-
AL049796.1
chr1
93847174
93848939
+
AL355075.4
chr14
20343048
20343685
-
DSCAM-AS1
chr21
40383083
40385358
+
KCNQ1OT1
chr11
2608328
2699994
-
LINC01772
chr1
16460948
16468481
+
MIR155HG
chr21
25561909
25575168
+
MIR663AHG
chr20
26167817
26251546
-
NEAT1
chr11
65422774
65445540
+
TUG1
chr22
30969245
30979395
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.