Entry Detail


General Information

Database ID:exR0258908
RNA Name:hsa-miR-6835-3p
RNA Type:miRNA
Chromosome:chr6
Starnd:+
Coordinate:
Start Site(bp):34240714End Site(bp):34240736
External Links:hsa-miR-6835-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
HMGA1
chr6
34236873
34246231
+
SASH1
chr6
148272304
148552048
+
HAPLN1
chr5
83637805
83720855
-
MAMDC2
chr9
70043848
70226972
+
PABPN1
chr14
23321289
23326185
+
PTPRF
chr1
43525187
43623666
+
PUM1
chr1
30931506
31065991
-
ZBTB32
chr19
35704558
35717038
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000591
chr15
41961025
41962156
+
hsa_circ_0000540
chr14
55799534
55821940
+
hsa_circ_0001168
chr20
47691321
47707559
+
hsa_circ_0000018
chr1
15860731
15863309
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC011815.1
chr18
35280867
35290201
-
AC020978.7
chr16
68224713
68227734
+
AC021078.1
chr5
149494314
149504670
-
AL137129.1
chr14
61734138
61776260
+
AL158825.2
chr9
75009828
75016036
-
FGD5-AS1
chr3
14920347
14948424
-
KCNQ1OT1
chr11
2608328
2699994
-
MIR17HG
chr13
91347820
91354579
+
MIR663AHG
chr20
26167817
26251546
-
NEAT1
chr11
65422774
65445540
+
OIP5-AS1
chr15
41283990
41309737
+
TERC
chr3
169764520
169765060
-
XIST
chrX
73820649
73852723
-
ZFAS1
chr20
49278178
49299600
+
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.