Entry Detail


General Information

Database ID:exR0259104
RNA Name:hsa-miR-7847-3p
RNA Type:miRNA
Chromosome:chr11
Starnd:+
Coordinate:
Start Site(bp):1880105End Site(bp):1880125
External Links:hsa-miR-7847-3p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
MAVS
chr20
3846799
3876123
+
ACTB
chr7
5527148
5563784
-
CRELD1
chr3
9933816
9945406
+
EVC
chr4
5711201
5814305
+
HNRNPUL2
chr11
62712630
62727457
-
HPRT1
chrX
134460165
134520513
+
MRPL35
chr2
86199355
86213790
+
MYC
chr8
127735434
127742951
+
PEX19
chr1
160276812
160286348
-
RPL10
chrX
154389955
154409168
+
RPS15
chr19
1438358
1440494
+
SLC25A6
chrX
1386152
1392113
-
SRRM2
chr16
2752626
2772538
+
TMSB4X
chrX
12975110
12977227
+
WIPF1
chr2
174559572
174682916
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:NA
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.