Entry Detail


General Information

Database ID:exR0259168
RNA Name:hsa-miR-877-5p
RNA Type:miRNA
Chromosome:chr6
Starnd:+
Coordinate:
Start Site(bp):30584332End Site(bp):30584351
External Links:hsa-miR-877-5p



Disease Information

Disease Name:Obesity
Disease Category:Other Diseases
MeSH ID:D009765
Type:Diseases Category/Nutritional and Metabolic Diseases
Alias:Appetite Depressants//Body Weight//Diet, Reducing//Skinfold Thickness//Lipectomy//Anti-Obesity Agents//Bariatrics



Expression Detail

GEO ID:GSE125494
Description:Adipocyte-Derived Exosomal microRNAs in subject serum samples
Experimental Design:Disease vs Control
Case Disease Type:Obesity
Case Disease SubType:NA
Case Sample:Obesity
Control Sample:Control
Number of Case:8
Number of Control:3
Number of Samples:11





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ABR
chr17
1003518
1229738
-
DNAJB6
chr7
157335381
157417439
+
EIF2AK1
chr7
6022247
6059175
-
FOSL1
chr11
65892049
65900573
-
IGF2BP1
chr17
48997385
49056145
+
MAPK8IP2
chr22
50600793
50613981
+
PHTF2
chr7
77798792
77957503
+
PLD3
chr19
40348456
40380439
+
SMG1
chr16
18804853
18926454
-
TRAF7
chr16
2155698
2178129
+
USP54
chr10
73497538
73625953
-
ANAPC7
chr12
110372900
110403730
-
EIF4ENIF1
chr22
31436977
31496108
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC010442.1
chr5
466124
473098
-
AC092718.4
chr16
81030770
81031485
+
DNAAF4-CCPG1
chr15
55355248
55498360
-
DSCAM-AS1
chr21
40383083
40385358
+
LINC00910
chr17
43338741
43389199
-
LINC01278
chrX
63222993
63561095
-
NEAT1
chr11
65422774
65445540
+
SNHG16
chr17
76557764
76565348
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE125494
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL21572
Method:Microarray
Num of detected RNA Type:1
Num of detected RNAs of this Type:2546
Sample treatment protocol:To isolate exosomes, we used the serum from lean and obese subejcts and used ExoQuick-TC precipitation solution, according to ExoQuick-TC protocol. We also used StemCell Technologies Magnetic Isolation Technique to isolate FABP4+ specific exosomes.
RNA Extract protocol:Total RNA was isolated following mirVana's total RNA Isolation kit.
RNA library preparation protocol:biotin We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit to label 1000 ng of cRNA from each sample



Reference

PMID:31331347
Title:Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs
Author:Barberio MD, Kasselman LJ, Playford MP, Epstein SB, Renna HA, Goldberg M, DeLeon J, Voloshyna I, Barlev A, Salama M, Ferrante SC, Nadler EP, Mehta N, Reiss AB, Freishtat RJ
Journal:J Transl Med. 2019 Jul 22;17(1):232.
Description:We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.