exRNAdisease

Entry Detail

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General Information

Database ID: exR0269307 RNA Name: hsa-miR-1248 RNA Type: miRNA Chromosome: chr3 Starnd: + Coordinate: Start Site(bp): 186786675 End Site(bp): 186786701 External Links: hsa-miR-1248

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CHD9 chr16 53055033 53329150 + ADAM10 chr15 58588809 58749791 - COA1 chr7 43608456 43729717 - COMT chr22 19941733 19969975 + COPB1 chr11 14443440 14500027 - EIF3L chr22 37848868 37889407 + EIF4A2 chr3 186783205 186789897 + GSPT1 chr16 11868128 11916082 - HNRNPA0 chr5 137745651 137754363 - INO80B chr2 74455087 74457944 + INO80B-WBP1 chr2 74455088 74460884 + KCTD10 chr12 109448655 109477359 - MRPL13 chr8 120380761 120445402 - RPS25 chr11 119015712 119018691 - SEC24B chr4 109433772 109540896 + SERINC5 chr5 80111651 80256048 - TM9SF2 chr13 99446311 99564048 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand SNHG1 chr11 62851984 62855953 - Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.