exRNAdisease

Entry Detail

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General Information

Database ID: exR0269393 RNA Name: hsa-miR-143-3p RNA Type: miRNA Chromosome: chr5 Starnd: + Coordinate: Start Site(bp): 149428978 End Site(bp): 149428998 External Links: hsa-miR-143-3p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACVR1B chr12 51951699 51997078 + BTF3L4 chr1 52056199 52090716 + BTG2 chr1 203305491 203309602 + CAPZB chr1 19338775 19485539 - CDK6 chr7 92604921 92836594 - CELSR2 chr1 109249539 109275751 + CIT chr12 119685791 119877320 - DEGS1 chr1 224175756 224193441 + DYRK1B chr19 39825350 39834201 - EFNA3 chr1 155078837 155087538 + FOSL2 chr2 28392448 28417317 + IGFBP5 chr2 216672105 216695549 - KRIT1 chr7 92198969 92246166 - MAP3K3 chr17 63622415 63696303 + NES chr1 156668763 156677407 - PACS1 chr11 66070272 66244744 + PATL1 chr11 59636716 59669037 - PNKD chr2 218270392 218346793 + RFWD3 chr16 74621399 74666877 - SLC7A11 chr4 138164097 138242349 - SON chr21 33543038 33577481 + STARD4 chr5 111496033 111512590 - STMN3 chr20 63639705 63657682 - TATDN2 chr3 10248023 10281218 + TFPI chr2 187464230 187565760 - TIGD5 chr8 143597831 143603224 + VPS37C chr11 61130257 61161615 - WSB1 chr17 27294076 27315926 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001247 chr22 46125304 46136418 + hsa_circ_0000711 chr16 68155889 68160513 + hsa_circ_0000431 chr12 102301963 102308010 + hsa_circ_0001168 chr20 47691321 47707559 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC009022.1 chr16 69976297 70065948 - AC091564.7 chr11 6610883 6616594 - AC245884.8 chr19 54438665 54439544 - DHRS4-AS1 chr14 23938219 24052555 - LINC00667 chr18 5237826 5290608 + LZTS1-AS1 chr8 20275773 20290458 + MALAT1 chr11 65497688 65506516 + MCM3AP-AS1 chr21 46229196 46259390 + OIP5-AS1 chr15 41283990 41309737 + Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.