exRNAdisease

Entry Detail

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General Information

Database ID: exR0269410 RNA Name: hsa-miR-149-5p RNA Type: miRNA Chromosome: chr2 Starnd: + Coordinate: Start Site(bp): 240456015 End Site(bp): 240456037 External Links: hsa-miR-149-5p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AMOTL2 chr3 134355874 134375479 - CDT1 chr16 88803789 88809258 + FOXP1 chr3 70952817 71583993 - FZD5 chr2 207762598 207769906 - GALNT2 chr1 230057990 230282122 + GNPTAB chr12 101745499 101830959 - ITGA11 chr15 68296532 68432163 - ITGA5 chr12 54395261 54419266 - MAP3K3 chr17 63622415 63696303 + MLLT1 chr19 6210381 6279975 - PHLPP2 chr16 71637835 71724701 - RAB31 chr18 9708275 9862551 + RCC2 chr1 17406760 17439677 - SLC6A6 chr3 14402576 14489349 + SMG5 chr1 156249224 156282825 - WDR36 chr5 111091716 111130502 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001731 chr7 100731990 100738448 + hsa_circ_0000241 chr10 70847874 70856944 + hsa_circ_0000907 chr19 17212469 17213367 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL035458.2 chr20 34234840 34281173 - AL160006.1 chr1 110058340 110062555 + MALAT1 chr11 65497688 65506516 + MIRLET7BHG chr22 46053869 46113928 + NEAT1 chr11 65422774 65445540 + SNHG8 chr4 118278709 118285316 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.