exRNAdisease

Entry Detail

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General Information

Database ID: exR0269577 RNA Name: hsa-miR-296-5p RNA Type: miRNA Chromosome: chr20 Starnd: - Coordinate: Start Site(bp): 58817661 End Site(bp): 58817681 External Links: hsa-miR-296-5p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACTN4 chr19 38647649 38731589 + CBX1 chr17 48070052 48101478 - EPHB4 chr7 100802565 100827523 - FOXP4 chr6 41546426 41602384 + GOSR2 chr17 46923075 46975524 + RFX1 chr19 13961530 14007039 - WDR5 chr9 134135365 134159968 + ZCCHC3 chr20 297570 300321 + GNL1 chr6 30541377 30557174 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016876.2 chr17 7581964 7584086 - AL132655.2 chr20 58817132 58817725 - AL355075.4 chr14 20343048 20343685 - AL356488.2 chr1 109100193 109100619 + KCNQ1OT1 chr11 2608328 2699994 - MIR34AHG chr1 9148011 9198906 - MIR663AHG chr20 26167817 26251546 - NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - STAG3L5P-PVRIG2P-PILRB chr7 100336104 100367831 + Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.