Entry Detail


General Information

Database ID:exR0269732
RNA Name:hsa-miR-3609
RNA Type:miRNA
Chromosome:chr7
Starnd:+
Coordinate:
Start Site(bp):98881700End Site(bp):98881723
External Links:hsa-miR-3609



Disease Information

Disease Name:Multiple Sclerosis
Disease Category:Nervous System Diseases
MeSH ID:D009103
Type:Diseases Category/Nervous System Diseases
Alias:Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating



Expression Detail

GEO ID:GSE140106
Description:Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment
Experimental Design:Fingolimod treatment
Case Disease Type:Multiple Sclerosis
Case Disease SubType:NA
Case Sample:Fingolimod treated Multiple Sclerosis
Control Sample:Untreated
Number of Case:27
Number of Control:27
Number of Samples:54





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
IGF2
chr11
2129112
2141238
-
MAP3K7
chr6
90513573
90587072
-
MYH9
chr22
36281277
36388067
-
PIK3C2A
chr11
17077730
17207983
-
CCNY
chr10
35247025
35572669
+
CCT2
chr12
69585426
69601570
+
GPRIN3
chr4
89236383
89307800
-
ING5
chr2
241687085
241729478
+
NUP98
chr11
3671083
3797792
-
P2RX5-TAX1BP3
chr17
3663063
3696194
-
PBX2
chr6
32184733
32190202
-
RPLP0
chr12
120196699
120201235
-
TAX1BP3
chr17
3662895
3668679
-
WDR74
chr11
62832342
62841809
-
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AL356488.2
chr1
109100193
109100619
+
Display:



Experiment Detail

GEO ID:GSE140106
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL11154
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:1907
Sample treatment protocol:Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science).
RNA Extract protocol:Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol.
RNA library preparation protocol:Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.



Reference

PMID:31721043
Title:Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment
Author:Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F
Journal:Mol Neurobiol. 2020 Feb;57(2):1245-1258.
Description:we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.