exRNAdisease

Entry Detail

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General Information

Database ID: exR0269818 RNA Name: hsa-miR-382-5p RNA Type: miRNA Chromosome: chr14 Starnd: + Coordinate: Start Site(bp): 101054316 End Site(bp): 101054337 External Links: hsa-miR-382-5p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand APCDD1 chr18 10454635 10489949 + BCAS2 chr1 114567557 114581629 - BCL2L11 chr2 111119378 111168445 + CAMTA1 chr1 6785454 7769706 + COBLL1 chr2 164653624 164843679 - ELK1 chrX 47635521 47650604 - FOSL1 chr11 65892049 65900573 - IGF2BP3 chr7 23310209 23470491 - KMT5A chr12 123383773 123409353 + MAPRE2 chr18 34976928 35143470 + NID1 chr1 235975830 236065109 - PARM1 chr4 74933095 75050115 + PDE8A chr15 84980440 85139145 + RAB18 chr10 27504174 27542237 + SCAF11 chr12 45919131 45992120 - SH3GL1 chr19 4360370 4400547 - STEAP3 chr2 119223831 119265652 + STK40 chr1 36339624 36385896 - TAGLN chr11 117199370 117207464 + TUB chr11 8019244 8106112 + UBN2 chr7 139230356 139308236 + WDR4 chr21 42843094 42879568 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC092718.4 chr16 81030770 81031485 + AC126365.1 chr17 20788071 20789584 + FGD5-AS1 chr3 14920347 14948424 - NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - TUG1 chr22 30969245 30979395 + Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.