Entry Detail


General Information

Database ID:exR0269847
RNA Name:hsa-miR-423-3p
RNA Type:miRNA
Chromosome:chr17
Starnd:+
Coordinate:
Start Site(bp):30117131End Site(bp):30117153
External Links:hsa-miR-423-3p



Disease Information

Disease Name:Multiple Sclerosis
Disease Category:Nervous System Diseases
MeSH ID:D009103
Type:Diseases Category/Nervous System Diseases
Alias:Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating



Expression Detail

GEO ID:GSE140106
Description:Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment
Experimental Design:Fingolimod treatment
Case Disease Type:Multiple Sclerosis
Case Disease SubType:NA
Case Sample:Fingolimod treated Multiple Sclerosis
Control Sample:Untreated
Number of Case:27
Number of Control:27
Number of Samples:54





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ATXN7
chr3
63864557
64003462
+
DCBLD2
chr3
98795941
98901695
-
ERP29
chr12
112013348
112023449
+
MGRN1
chr16
4616493
4690974
+
SH3GLB2
chr9
129007036
129028303
-
SLC7A1
chr13
29509414
29595688
-
ENO1
chr1
8861000
8879190
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001866
chr9
86293355
86297981
-
hsa_circ_0001865
chr9
86292641
86293514
-
lncRNA targets:NA
Display:



Experiment Detail

GEO ID:GSE140106
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL11154
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:1907
Sample treatment protocol:Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science).
RNA Extract protocol:Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol.
RNA library preparation protocol:Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.



Reference

PMID:31721043
Title:Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment
Author:Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F
Journal:Mol Neurobiol. 2020 Feb;57(2):1245-1258.
Description:we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.