exRNAdisease

Entry Detail

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General Information

Database ID: exR0269918 RNA Name: hsa-miR-454-3p RNA Type: miRNA Chromosome: chr17 Starnd: - Coordinate: Start Site(bp): 59137787 End Site(bp): 59137809 External Links: hsa-miR-454-3p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TARDBP chr1 11012344 11026420 + PPP1R9A chr7 94907202 95296415 + KIAA0232 chr4 6781375 6884170 + ITGB8 chr7 20330702 20415754 + PHF20 chr20 35771974 35950370 + ATP6V1C1 chr8 103021063 103073051 + CALU chr7 128739292 128773400 + CIT chr12 119685791 119877320 - G6PD chrX 154531391 154547572 - CHD9 chr16 53055033 53329150 + BHLHE40 chr3 4979437 4985323 + ADARB1 chr21 45073853 45226560 + SLC12A7 chr5 1050384 1112063 - EIF5A2 chr3 170888418 170908644 - TGIF2 chr20 36573488 36593950 + AKAP10 chr17 19904302 19978343 - MLPH chr2 237485428 237555322 + CD151 chr11 832887 839831 + LDLRAD3 chr11 35943981 36232136 + UBQLN1 chr9 83659968 83707958 - APCDD1 chr18 10454635 10489949 + SYNJ1 chr21 32628759 32728048 - MPPED2 chr11 30384493 30586872 - LYSMD2 chr15 51723011 51751585 - PRICKLE2 chr3 64092236 64445476 - MLLT1 chr19 6210381 6279975 - RTN4 chr2 54972187 55112621 - MTMR9 chr8 11284816 11328146 + MAP3K20 chr2 173075435 173268015 + SH3PXD2A chr10 103594027 103855543 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000591 chr15 41961025 41962156 + hsa_circ_0001454 chr4 154524454 154533552 + hsa_circ_0000384 chr12 27867712 27877119 + hsa_circ_0001723 chr7 91924202 91948826 + hsa_circ_0000892 chr19 11941431 12014514 + hsa_circ_0001605 chr6 42903447 42905047 + hsa_circ_0000155 chr1 168007608 168014465 + hsa_circ_0001495 chr5 68470703 68471364 + hsa_circ_0000943 chr19 47421744 47440665 + hsa_circ_0000018 chr1 15860731 15863309 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC011503.2 chr19 23927788 23929287 + AC021092.1 chr19 44103007 44113183 - AC135050.6 chr16 31122235 31124064 + AC234582.1 chr1 155195004 155205495 + AL031595.3 chr22 44139365 44153626 + AL049840.4 chr14 103694516 103695050 - AL158206.1 chr9 19453209 19455173 + AL158825.2 chr9 75009828 75016036 - AL603839.3 chr1 40493157 40508661 - CKMT2-AS1 chr5 81201341 81301565 - H19 chr11 1995176 2001470 - HAGLR chr2 176164051 176188958 - KCNQ1OT1 chr11 2608328 2699994 - LINC01089 chr12 121795267 121803906 - MIR17HG chr13 91347820 91354579 + NEAT1 chr11 65422774 65445540 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.