exRNAdisease

Entry Detail

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General Information

Database ID: exR0270069 RNA Name: hsa-miR-493-3p RNA Type: miRNA Chromosome: chr14 Starnd: + Coordinate: Start Site(bp): 100869116 End Site(bp): 100869137 External Links: hsa-miR-493-3p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ANKS1A chr6 34889255 35091406 + ASF1B chr19 14119512 14136613 - BACH2 chr6 89926528 90296908 - BRPF3 chr6 36196744 36232790 + C17orf49 chr17 7014495 7017525 + DYNLRB1 chr20 34516409 34540958 + EPG5 chr18 45847609 45967329 - HIF1AN chr10 100529072 100559998 + MEX3A chr1 156072013 156082465 - MFN2 chr1 11980181 12013514 + MMGT1 chrX 135962070 135974063 - MTUS1 chr8 17643795 17800917 - NPTX1 chr17 80466834 80477843 - PRKAR2A chr3 48744597 48847874 - PXN chr12 120210439 120265771 - RNASEK-C17orf49 chr17 7012635 7017520 + XPO6 chr16 28097979 28211920 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001146 chr20 34241449 34246936 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016831.1 chr7 130876809 130913310 - AC016876.2 chr17 7581964 7584086 - AC022167.2 chr16 8848105 8860456 + AC144548.1 chr12 110387463 110445548 - AL359924.1 chr1 237862175 237928321 + MIR200CHG chr12 6963246 6964447 + TUG1 chr22 30969245 30979395 + Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.