exRNAdisease

Entry Detail

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General Information

Database ID: exR0270115 RNA Name: hsa-miR-5094 RNA Type: miRNA Chromosome: chr15 Starnd: - Coordinate: Start Site(bp): 89850690 End Site(bp): 89850711 External Links: hsa-miR-5094

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ZNHIT3 chr17 36486629 36499310 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001454 chr4 154524454 154533552 + hsa_circ_0001005 chr2 53921020 53978078 - hsa_circ_0000384 chr12 27867712 27877119 + hsa_circ_0001159 chr20 40161688 40179999 - hsa_circ_0000698 chr16 47531309 47549512 + hsa_circ_0001445 chr4 144464661 144465125 + hsa_circ_0000943 chr19 47421744 47440665 + hsa_circ_0001667 chr7 810107 814799 + hsa_circ_0001376 chr3 194169185 194175121 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC074117.1 chr2 27356246 27367622 + AL356488.2 chr1 109100193 109100619 + AL359091.1 chr9 128392007 128393510 - DSCAM-AS1 chr21 40383083 40385358 + FTX chrX 73940435 74293574 - GAS5 chr1 173858559 173868882 - NEAT1 chr11 65422774 65445540 + NUTM2B-AS1 chr10 79661394 79826594 - PDCD4-AS1 chr10 110869868 110872233 - SNHG3 chr1 28505980 28510892 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.