exRNAdisease

Entry Detail

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General Information

Database ID: exR0270124 RNA Name: hsa-miR-516b-5p RNA Type: miRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 53736860 End Site(bp): 53736881 External Links: hsa-miR-516b-5p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand BCL2L11 chr2 111119378 111168445 + CHKB-CPT1B chr22 50568869 50582965 - CPT1B chr22 50568861 50578465 - DNAJC9 chr10 73183362 73247255 - FAM13B chr5 137937960 138051961 - FNBP4 chr11 47716494 47767443 - FOSL2 chr2 28392448 28417317 + FSD1 chr19 4304598 4323843 + GOLGA1 chr9 124878275 124948492 - HIF1AN chr10 100529072 100559998 + MAK16 chr8 33485182 33501262 + NEMP1 chr12 57055643 57088063 - NID1 chr1 235975830 236065109 - PIGS chr17 28553383 28571794 - QSER1 chr11 32892820 32993316 + RABGAP1 chr9 122940833 123104866 + S100A16 chr1 153606886 153613145 - SGPP1 chr14 63684216 63728065 - STAT3 chr17 42313324 42388568 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000698 chr16 47531309 47549512 + hsa_circ_0001727 chr7 99621041 99621930 + hsa_circ_0001495 chr5 68470703 68471364 + hsa_circ_0000825 chr18 8718421 8720494 + hsa_circ_0001387 chr4 1902352 1936989 + hsa_circ_0000038 chr1 28800065 28802803 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016876.2 chr17 7581964 7584086 - AC079781.5 chr7 97851688 97972985 - AL157392.3 chr10 13631143 13668445 + COL4A2-AS2 chr13 110456396 110463287 - LINC00324 chr17 8220642 8224043 - LINC01184 chr5 127939152 128083172 - MIR194-2HG chr11 64889560 64893449 - NEAT1 chr11 65422774 65445540 + PCBP1-AS1 chr2 69962263 70103220 - TBC1D3P1-DHX40P1 chr17 59962363 60018975 - TUG1 chr22 30969245 30979395 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.