exRNAdisease

Entry Detail

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General Information

Database ID: exR0270160 RNA Name: hsa-miR-525-5p RNA Type: miRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 53697547 End Site(bp): 53697567 External Links: hsa-miR-525-5p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand C4orf46 chr4 158666675 158672255 - CA12 chr15 63321378 63381846 - CELSR2 chr1 109249539 109275751 + CHD1 chr5 98853985 98928957 - HMOX1 chr22 35380361 35394207 + ITGA5 chr12 54395261 54419266 - KIAA0232 chr4 6781375 6884170 + LRP8 chr1 53242364 53328469 - NCEH1 chr3 172630249 172711218 - ORMDL3 chr17 39921041 39927601 - PRRC2B chr9 131373636 131500197 + SORBS3 chr8 22544986 22575788 + SSH2 chr17 29625938 29930276 - TGOLN2 chr2 85318020 85328296 - USP16 chr21 29024629 29054488 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000892 chr19 11941431 12014514 + hsa_circ_0001361 chr3 171830241 171851336 + hsa_circ_0001146 chr20 34241449 34246936 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC079781.5 chr7 97851688 97972985 - AL049543.1 chr6 28587378 28591747 + AL049795.2 chr1 32170733 32176568 + GAS5 chr1 173858559 173868882 - H19 chr11 1995176 2001470 - HCP5 chr6 31463170 31478936 + MIRLET7BHG chr22 46053869 46113928 + NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - XIST chrX 73820649 73852723 - ZFAS1 chr20 49278178 49299600 + Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.