exRNAdisease

Entry Detail

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General Information

Database ID: exR0270167 RNA Name: hsa-miR-541-3p RNA Type: miRNA Chromosome: chr14 Starnd: + Coordinate: Start Site(bp): 101064548 End Site(bp): 101064569 External Links: hsa-miR-541-3p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand REPIN1 chr7 150368189 150374044 + HOXD11 chr2 176104216 176109754 + LY6E chr8 143017982 143023832 + FASN chr17 82078338 82098294 - M6PR chr12 8940361 8949761 - TOM1L2 chr17 17843511 17972422 - ACACA chr17 37084992 37406836 - TAF4 chr20 61953469 62065810 - MID1IP1 chrX 38801432 38806537 + FBXO46 chr19 45710629 45730896 - BICRA chr19 47608196 47703277 + CALR chr19 12938578 12944489 + ARHGDIA chr17 81867721 81871378 - UCP2 chr11 73974672 73983246 - FKBP1A chr20 1368978 1393172 - SLC6A6 chr3 14402576 14489349 + NFIX chr19 12995608 13098796 + ADGRL1 chr19 14147743 14206187 - NID1 chr1 235975830 236065109 - CAPZB chr1 19338775 19485539 - AGRN chr1 1020120 1056118 + AXL chr19 41219223 41261766 + MAFG chr17 81918270 81927735 - PKM chr15 72199029 72231822 - IGFBP5 chr2 216672105 216695549 - MIGA2 chr9 129036621 129072082 + ZMIZ1 chr10 79068966 79316528 + PRRC2B chr9 131373636 131500197 + ACVR1B chr12 51951699 51997078 + DMWD chr19 45782947 45792845 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000417 chr12 69644908 69656342 + hsa_circ_0000642 chr15 80390757 80415142 + hsa_circ_0000816 chr17 80521229 80526077 + hsa_circ_0000643 chr15 80412669 80415142 + hsa_circ_0000943 chr19 47421744 47440665 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC239868.1 chr1 149861271 149862504 + AL035458.2 chr20 34234840 34281173 - AL356488.2 chr1 109100193 109100619 + NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - OIP5-AS1 chr15 41283990 41309737 + STAG3L5P-PVRIG2P-PILRB chr7 100336104 100367831 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.