exRNAdisease

Entry Detail

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General Information

Database ID: exR0270289 RNA Name: hsa-miR-642a-5p RNA Type: miRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 45674943 End Site(bp): 45674964 External Links: hsa-miR-642a-5p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AFF4 chr5 132875395 132963634 - ARID3A chr19 925781 975939 + E2F3 chr6 20401879 20493714 + GFOD1 chr6 13357830 13487662 - HIF1AN chr10 100529072 100559998 + MAP3K12 chr12 53479669 53500063 - MAPKAPK2 chr1 206684944 206734283 + MDK chr11 46380756 46383837 + PPP6R1 chr19 55229779 55259017 - SIK2 chr11 111602449 111730855 + SLC7A2 chr8 17497088 17570573 + SYVN1 chr11 65121780 65134533 - TMED10 chr14 75131469 75176612 - TMEM30B chr14 61277370 61281761 - UBR3 chr2 169827454 170084131 + VAT1 chr17 43014607 43025123 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001005 chr2 53921020 53978078 - hsa_circ_0001146 chr20 34241449 34246936 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004832.5 chr22 30420512 30420912 + AC007952.4 chr17 19112000 19112636 - AC010327.5 chr19 55216660 55221616 + AC016876.2 chr17 7581964 7584086 - AL645465.1 chr1 244375100 244409592 - MIR34AHG chr1 9148011 9198906 - MIR497HG chr17 7015818 7019659 - NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - ZFAS1 chr20 49278178 49299600 + Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.