| GEO ID: | GSE140106 |
| Sample Source: | Blood |
| Source Fraction: | Exosome |
| Platform: | GPL11154 |
| Method: | NGS |
| Num of detected RNA Type: | 1 |
| Num of detected RNAs of this Type: | 1907 |
| Sample treatment protocol: | Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). |
| RNA Extract protocol: | Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. |
| RNA library preparation protocol: | Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions. |