GEO ID: | GSE140144 |
Sample Source: | Blood |
Source Fraction: | Serum |
Platform: | GPL18573 |
Method: | NGS |
Num of detected RNA Type: | 1 |
Num of detected RNAs of this Type: | 614 |
Sample treatment protocol: | Total RNA samples are pretreated to remove some RNA modifications that interfere with small RNA-seq library construction. |
RNA Extract protocol: | Total RNA from patients' sera were isolated with TRIzol LS Reagent (Life Technologies, USA), following the manufacturer's protocols. |
RNA library preparation protocol: | 134-160 bp PCR amplified fragments were extracted and purified from the PAGE gel. |