GEO ID: | GSE94519 |
Sample Source: | Blood |
Source Fraction: | Serum |
Platform: | GPL20115 |
Method: | Microarray |
Num of detected RNA Type: | 1 |
Num of detected RNAs of this Type: | 1224 |
Sample treatment protocol: | All blood samples were separately collected and sequentially centrifuged at 2000 rpm for 10 min. The cell-free serum from the supernatant was stored at -80°C for further detection. |
RNA Extract protocol: | RNA was prepared using the Versagene Blood RNA Purification kit (Gentra Systems, Minneapolis MN). |
RNA library preparation protocol: | Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). |