exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359181 RNA Name: AAAS RNA Type: mRNA Chromosome: chr12 Starnd: - Coordinate: Start Site(bp): 53307456 End Site(bp): 53324864 External Links: ENSG00000094914

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand MSI1 chr12 120341330 120369164 - RPL11 chr1 23691742 23696835 + RPLP2 chr11 809965 812880 + MIF chr22 23894383 23895227 + MRPS10 chr6 42206807 42217861 - RCSD1 chr1 167630093 167708696 + LRCH4 chr7 100574011 100586129 - LRP2 chr2 169127109 169362534 - MEX3C chr18 51174550 51218304 - MTRNR2L12 chr3 96617188 96618236 - MTA1 chr14 105419820 105470729 + B2M chr15 44711487 44718877 + CDC25B chr20 3786772 3806121 + SFPQ chr1 35176378 35193145 - ZNF207 chr17 32350117 32381886 + VPS41 chr7 38722974 38932394 - RPL13 chr16 89560657 89566828 + METTL16 chr17 2405562 2511891 - MAPK14 chr6 36027677 36111236 + CD99 chrX 2691187 2741309 + YBX3 chr12 10699089 10723323 - AP000350.4 chr22 23862188 23895223 + PKM chr15 72199029 72231822 - FRAS1 chr4 78057323 78544269 + LRIG1 chr3 66378797 66501263 - RACK1 chr5 181236897 181248096 - ALDOA chr16 30064164 30070457 + MYL6 chr12 56158161 56163496 + NBR1 chr17 43170481 43211689 + LAMA5 chr20 62307955 62367312 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC069281.2 chr7 100572232 100578700 - PCAT6 chr1 202810954 202812156 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.